Enterocytozoon bieneusi (microsporidia) in faecal samples from domestic animals from Galicia, Spain

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Enterocytozoon bieneusi (microsporidia) in faecal samples from domestic animals from Galicia, Spain.

In this survey we examined 87 domestic animal stool samples in order to detect the possible presence of microsporidia in animals in close contact with humans in Galicia (NW, Spain). The detection of Enterocytozoon bieneusi spores was confirmed in faecal samples from two dogs and one goat by polymerase chain reaction. None of the positive samples for microsporidia in the staining method were amp...

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Correction: Correction: Microsporidia Detection and Genotyping Study of Human Pathogenic E. bieneusi in Animals from Spain

Microsporidia are ubiquitous parasites infecting all animal phyla and we present evidence that supports their zoonotic potential. Fecal samples taken from domestic (cats and dogs), farm (pigs, rabbits and ostriches) and wild animals (foxes) from different provinces of Spain were evaluated for microsporidia infection by light microscopy and PCR. After Microsporidia species identification, E. bie...

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Correction: Microsporidia Detection and Genotyping Study of Human Pathogenic E. bieneusi in Animals from Spain

Copyright: ß 2014 The PLOS ONE Staff. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Diagnosis of Enterocytozoon bieneusi by PCR in stool samples eluted from filter paper disks.

We report a PCR-based assay for the detection of Enterocytozoon bieneusi. We extracted DNA from feces which had been applied to filter paper disks and evaluated four preserving solutions. Infected specimens were identified by electrophoresis of amplicons from concentrated formalin-fixed samples and unconcentrated fresh feces. Our findings demonstrate that this methodology is effective for sampl...

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Genetic diversity in Enterocytozoon bieneusi from dogs and cats

24 To explore the genetic diversity, host specificity and zoonotic potential of Enterocytozoon 25 bieneusi, feces from 348 stray and pet dogs, and 96 pet cats from different locations in China 26 were examined by internal transcribed spacer (ITS) based PCR. E. bieneusi was detected in 27 15.5% of dogs including 20.5% of stray dogs and 11.7% of pet dogs, and in 11.5% of pet 28 cats. Higher infec...

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ژورنال

عنوان ژورنال: Memórias do Instituto Oswaldo Cruz

سال: 2002

ISSN: 0074-0276

DOI: 10.1590/s0074-02762002000700003